thr77 - Human pCyclin E1 Thr77 Detection Kit goofy ahh ALSUPCYCE1A10KALSUPCYCE1A50K ALSUPCYCE1AHVKit specificityThis assay kit contains antibodies whic recognize the phosphoThr77 epitope and a distal epitope on Cycli E1 The protein detected by this kit corresponds to UniProt D P24864 Cyclin E1 is also known as G1Sspecific cy Cyclin E1 PhosphoThr77 Antibody AAT Bioquest Contact tech support Invitrogen AntiPhosphoCyclin E Thr77 Polyclonal Catalog PA5104585 Tested in Western Blot WB Immunocytochemistry ICCIF and Immunohistochemistry Paraffin IHC P applications This antibody reacts with Human Mouse Rat samples Supplied as 100 µL purified antibody 1 mgmL ImmunohistochemistryParaffin Cyclin E1 p Thr77 Antibody SD2025 NBP267760 Analysis of paraffinembedded human colon cancer tissue using antiPhosphoCyclin E1 T77 antibody Counter stained with hematoxylin ImmunohistochemistryParaffin Cyclin E1 p Thr77 Antibody SD2025 NBP267760 Analysis of paraffinembedded human tonsil Contact tech support AbboMax AntiPhosphoDesmin Thr76 Thr77 Polyclonal Catalog 602280 Tested in Western Blot WB and Immunohistochemistry Paraffin IHC P applications This antibody reacts with Human Mouse Rat samples Supplied as 100 µg purified antibody 05 mgmL AlphaLISA SureFire Ultra Human PhosphoCyclin E1 Thr77 View Rabbit Monoclonal antiCyclin E1 p Thr77 Antibody SR1828 NBP321555 Validated Applications WB ICCIF IHC Validated Species Human PhosphoCyclin E Thr77 Polyclonal Antibody PA5104585 Cyclin E1 p Thr77 Antibody SR1828 Novus Biologicals Cyclin E1 PhosphoThr77 Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr77 Formulation Rabbit IgG in phosphate buffered saline without Mg2 and Ca2 pH 74 150mM NaCl 002 sodium azide and 50 glycerol Cyclin E1 p Thr77 Antibody SD2025 Novus Biologicals Desmin Thr76 Thr77 Antibody Bioss Samples were incubated with PhosphoCyclin E Thr77 Monoclonal antibody Product MA538232 using a dilution of 11000 followed by HRP Goat AntiRabbit IgG HL secondary antibody at a dilution of 110000 NIH3T3 cells were treated by Hydroxyurea 4 mM at 37C for 20 hours Lysatesproteins 25 µg per lane PhosphoCyclin E Thr77 Recombinant Monoclonal Antibody Homeodomaininteracting Protein Kinase2 HIPK2 Chemical screening identifies ATM as a target for alleviating The AlphaLISA SureFire Human PhosphoCyclin E1 Thr77 Detection Kit is a sandwich immunoassay for the quantitative detection of phosphoCyclin E1 Thr77 in cellular lysates using Alpha Technology with a cyclin E1 phosphoThr77 antibody that recognizes Cyclin E1 of human origin Formats AlphaLISA SureFire Ultra Human PhosphoCyclin E1 Thr77 AlphaLISA SureFire Ultra Human pCyclin E1 Thr77 PECy7 Rabbit KLH conjugated synthetic phosphopeptide derived from human DES around the phosphorylation site of Thr76Thr77 Thr76 Thr77 Polyclonal IgG 1ugul Purified by Protein A Aqueous buffered solution containing 001M TBS pH74 with 1 BSA 003 Proclin300 and 50 Glycerol Conjugation We provide custom conjugation services tambang888 for this antibody eg labeling of Cyclin E1 PhosphoThr77 Antibody with HRP A list of available labels can be found in the table below AF AF350 AF488 AF555 AF594 AF647 AF680 AF700 AF750 Proteins PhosphoDesmin Thr76 Thr77 Polyclonal Antibody BS5302R This control lysate is intended for use with the AlphaLISA SureFire Ultra Human PhosphoCyclin E1 Thr77 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay Desmin Thr76 Thr77 Antibody PECy7 Conjugated Bioss Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle These proteins have been well conserved through evolution and play a critical role in regulation of cell division cyclin E along with the three cyclin D proteins and cyclin C has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression Contact tech support Bioss AntiPhosphoDesmin Thr76 Thr77 Polyclonal Catalog BS5302R Tested in Western Blot WB Immunohistochemistry Paraffin IHC P Flow Cytometry Flow and ELISA ELISA applications This antibody reacts with Human samples Supplied as 100 µL purified antibody 1 mgmL Cyclin E1 p Thr77 Antibody EPR22422 Novus Biologicals Paraformaldehydefixed paraffin embedded human heart Antigen retrieval by boiling in sodium citrate buffer pH60 for 15min Block endogenous peroxidase by 3 hydrogen peroxide for 20 minutes Blocking buffer normal goat serum at 37C for 30min Incubation with Desmin Thr76 Thr77 Polyclonal Antibody Unconjugated bs5302R at 1200 overnight at 4C followed by Cyclin E Thr77 Polyclonal Antibody PECy7 Conjugated Cyclin E1 Antibody PhosphoThr77 OAAF07468 Aviva PhosphoCyclin E Thr77 Recombinant Monoclonal Antibody AntiCCNE1 phosphoThr77 Rabbit Polyclonal Antibody VWR The chromosomal highmobility group A HMGA proteins comprising of HMGA1a HMGA1b and HMGA2 play important roles in the regulation of numerous processes in eukaryotic cells such as transcriptional regulation DNA repair RNA processing and chromatin PhosphoDesmin Thr76 Thr77 Polyclonal Antibody 602280 Thr77 Clonality Polyclonal Isotype IgG Concentration 1ugul Purification Purified by Protein A Storage Buffer Aqueous buffered solution containing 001M TBS We then assessed whether ATP6V1G1 Thr77 phosphorylation is critical for regulation of V 1domain assembly ATP6V1G1 WT and Esubunit interaction was decreased by ETO treatment but was restored Contact tech support Invitrogen AntiPhosphoCyclin E Thr77 Recombinant Monoclonal SD2025 Catalog MA532358 Tested in Western Blot WB Immunocytochemistry ICCIF and Immunohistochemistry Paraffin IHC P applications This antibody reacts with Human samples Supplied as 100 µL purified antibody 1 mgmL View Rabbit Monoclonal antiCyclin E1 p Thr77 Antibody EPR22422 NBP196107 Validated Applications WB ICCIF IHC gigs adalah IHCP Validated Species Human
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